What is a scanning electron microscope used for?
Because of its great depth of focus, a scanning electron microscope is the EM analog of a stereo light microscope. It provides detailed images of the surfaces of cells and whole organisms that are not possible by TEM. It can also be used for particle counting and size determination, and for process control.
What is the principle of SEM?
The Scanning electron microscope works on the principle of applying kinetic energy to produce signals on the interaction of the electrons. These electrons are secondary electrons, backscattered electrons, and diffracted backscattered electrons which are used to view crystallized elements and photons.
What is the difference between SEM and TEM?
The difference between SEM and TEM The main difference between SEM and TEM is that SEM creates an image by detecting reflected or knocked-off electrons, while TEM uses transmitted electrons (electrons that are passing through the sample) to create an image.
Can viruses be seen with an electron microscope?
Viruses are very small and most of them can be seen only by TEM (transmission electron microscopy). TEM has therefore made a major contribution to virology, including the discovery of many viruses, the diagnosis of various viral infections and fundamental investigations of virus-host cell interactions.
Why is SEM useful?
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How do you use a SEM microscope?
0:187:16Using a Scanning Electron Microscope – YouTubeYouTube
What are the advantages of SEM?
SEM Advantages Advantages of a Scanning Electron Microscope include its wide-array of applications, the detailed three-dimensional and topographical imaging and the versatile information garnered from different detectors.