Staph latex test procedure

Staph latex test procedure

How does the staph latex test work?

This staph latex test is a slide agglutination assay for the qualitative detection of coagulase (both clumping factor and protein A) to identify Staphylococcus aureus to the exclusion of other species of staphylococci. For use on pure culture samples suspected of being S. aureus. It detects methicillin-resistant S.

What is the significance of getting a positive result after performing the Staphyloslide test?

The result is positive when there is noticeable clearing of the blue background in the test latex. This indicates the presence of S. aureus.

How does Bactistaph test work?

Use this rapid test utilizing latex particles which are capable of detecting both clumping factor and protein A simultaneously. Agglutination of white latex particles is visible to the unaided eye within 60 seconds and represents a positive reaction.

How do you test for Staphylococcus aureus?

Most often, doctors diagnose staph infections by checking a tissue sample or nasal secretions for signs of the bacteria. Other tests. If you're diagnosed with a staph infection, your doctor may order an imaging test called an echocardiogram to check if the infection has affected your heart.

How does latex agglutination test work?

The test depends on what type of sample is needed. The sample is sent to a lab, where it is mixed with latex beads coated with a specific antibody or antigen. If the suspected substance is present, the latex beads will clump together (agglutinate). Latex agglutination results take about 15 minutes to an hour.

Why coagulase test is done?

Coagulase test is used to differentiate Staphylococcus aureus (positive) which produce the enzyme coagulase, from S. epidermis and S. saprophyticus (negative) which do not produce coagulase. i.e Coagulase Negative Staphylococcus (CONS).

What component of the staphylococcal cell is detected in the latex test?

The Staphaurex kit is an agglutination test that detects the presence of bound coagulase and protein A. The test reagent is composed of latex particles coated with purified human IgG and fibrinogen.